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    Quiescent epithelial cell rests of Malassez can differentiate into ameloblast-like cells.

    Authors
    Shinmura Y, Tsuchiya S, Hata K, et al. 
    Institution

    Division of Molecular and Developmental Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

    Source
    J Cell Physiol 2008 Dec; 217(3) :728-38.
    Abstract

    Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. After completion of crown formation, HERS are converted from cervical loop cells, which have the potential to generate enamel for tooth crown formation. Cervical loop cells have the potential to differentiate into ameloblasts. Generally, no new ameloblasts can be generated from HERS, however this study demonstrated that subcultured ERM can differentiate into ameloblast-like cells and generate enamel-like tissues in combination with dental pulp cells at the crown formation stage. Porcine ERM were obtained from periodontal ligament tissue by explant culture and were subcultured with non-serum medium. Thereafter, subcultured ERM were expanded on 3T3-J2 feeder cell layers until the tenth passage. The in vitro mRNA expression pattern of the subcultured ERM after four passages was found to be different from that of enamel organ epithelial cells and oral gingival epithelial cells after the fourth passage using the same expansion technique. When subcultured ERM were combined with subcultured dental pulp cells, ERM expressed cytokeratin14 and amelogenin proteins in vitro. In addition, subcultured ERM combined with primary dental pulp cells seeded onto scaffolds showed enamel-like tissues at 8 weeks post-transplantation. Moreover, positive staining for amelogenin was observed in the enamel-like tissues, indicating the presence of well-developed ameloblasts in the implants. These results suggest that ERM can differentiate into ameloblast-like cells.

    Mesh
    3T3 Cells
    Ameloblasts
    Amelogenin
    Animals
    Cell Differentiation
    Cell Proliferation
    Cell Separation
    Cell Transplantation
    Cells, Cultured
    Collagen
    Dental Enamel
    Dental Pulp
    Epithelial Cells
    Gene Expression Regulation
    Incisor
    Keratin-14
    Mice
    Periodontal Ligament
    Rats
    Reverse Transcriptase Polymerase Chain Reaction
    Swine
    Tooth, Deciduous
    Language

    eng

    Pub Type(s)
    Journal Article Research Support, Non-U.S. Gov't
    PubMed ID

    18663726

    Content Manager
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