MEDLINE Journals

    Simultaneous determination of irbesartan and hydrochlorothiazide in human plasma using HPLC coupled with tandem mass spectrometry: Application to bioequivalence studies.

    Authors
    Tutunji LF, Tutunji MF, Alzoubi MI, et al. 
    Source
    J Pharm Biomed Anal 2010 Mar 11; 51(4) :985-90.
    Abstract

    A sensitive, specific and selective liquid chromatography/tandem mass spectrometric method has been developed and validated for the simultaneous determination of irbesartan and hydrochlorothiazide in human plasma. Plasma samples were prepared using protein precipitation with acetonitrile, the two analytes and the internal standard losartan were separated on a reverse phase C(18) column (50mmx4mm, 3microm) using water with 2.5% formic acid, methanol and acetonitrile (40:45:15, v/v/v (%)) as a mobile phase (flow rate of 0.70mL/min). Irbesartan and hydrochlorothiazide were ionized using ESI source in negative ion mode, prior to detection by multiple reaction monitoring (MRM) mode while monitoring at the following transitions: m/z 296-->269 and m/z 296-->205 for hydrochlorothiazide, 427-->175 for irbesartan. Linearity was demonstrated over the concentration range 0.06-6.00microg/mL for irbesartan and 1.00-112.00ng/mL for hydrochlorothiazide. The developed and validated method was successfully applied to a bioequivalence study of irbesartan (300mg) with hydrochlorothiazide (12.5mg) tablet in healthy volunteers (N=36).

    Mesh
    Administration, Oral
    Adolescent
    Adult
    Angiotensin II Type 1 Receptor Blockers
    Biphenyl Compounds
    Calibration
    Chemical Precipitation
    Chromatography, High Pressure Liquid
    Cross-Over Studies
    Diuretics
    Drug Combinations
    Drug Stability
    Humans
    Hydrochlorothiazide
    Male
    Middle Aged
    Reproducibility of Results
    Spectrometry, Mass, Electrospray Ionization
    Tablets
    Tandem Mass Spectrometry
    Tetrazoles
    Therapeutic Equivalency
    Young Adult
    Language

    eng

    Pub Type(s)
    Journal Article Randomized Controlled Trial Validation Studies
    PubMed ID

    20004542

    Content Manager
    Related Content

    Simultaneous determination of amiloride and hydrochlorothiazide in human plasma by liquid chromatography/tandem mass spectrometry with positive/negative ion-switching electrospray ionisation.

    Simultaneous determination of bisoprolol and hydrochlorothiazide in human plasma by HPLC coupled with tandem mass spectrometry.

    Determination of hydrochlorothiazide in human plasma by liquid chromatography/tandem mass spectrometry.

    A liquid chromatography/tandem mass spectrometry method for the simultaneous quantification of valsartan and hydrochlorothiazide in human plasma.

    Hydrophilic interaction chromatography-tandem mass spectrometric analysis of irbesartan in human plasma: application to pharmacokinetic study of irbesartan.

    Liquid chromatographic-tandem mass spectrometric method for the simultaneous quantitation of telmisartan and hydrochlorothiazide in human plasma.

    High performance liquid chromatography/negative ion electrospray tandem mass spectrometry method for the measurement of hydrochlorothiazide in human plasma: application to a comparative bioavailability study.

    HPLC determination of irbesartan in human plasma: its application to pharmacokinetic studies.