MEDLINE Journals

    Growth and ethanol fermentation ability on hexose and pentose sugars and glucose effect under various conditions in thermotolerant yeast Kluyveromyces marxianus.

    Authors
    Rodrussamee N, Lertwattanasakul N, Hirata K, et al. 
    Institution

    Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan.

    Source
    Appl Microbiol Biotechnol 2011 May; 90(4) :1573-86.
    Abstract

    Ethanol fermentation ability of the thermotolerant yeast Kluyveromyces marxianus, which is able to utilize various sugars including glucose, mannose, galactose, xylose, and arabinose, was examined under shaking and static conditions at high temperatures. The yeast was found to produce ethanol from all of these sugars except for arabinose under a shaking condition but only from hexose sugars under a static condition. Growth and sugar utilization rate under a static condition were slower than those under a shaking condition, but maximum ethanol yield was slightly higher. Even at 40°C, a level of ethanol production similar to that at 30°C was observed except for galactose under a static condition. Glucose repression on utilization of other sugars was observed, and it was more evident at elevated temperatures. Consistent results were obtained by the addition of 2-deoxyglucose. The glucose effect was further examined at a transcription level, and it was found that KmGAL1 for galactokinase and KmXYL1 for xylose reductase for galactose and xylose/arabinose utilization, respectively, were repressed by glucose at low and high temperatures, but KmHXK2 for hexokinase was not repressed. We discuss the possible mechanism of glucose repression and the potential for utilization of K. marxianus in high-temperature fermentation with mixed sugars containing glucose.

    Mesh
    Bacterial Proteins
    Ethanol
    Fermentation
    Glucose
    Hexoses
    Hot Temperature
    Kluyveromyces
    Pentoses
    Language

    eng

    Pub Type(s)
    Journal Article Research Support, Non-U.S. Gov't
    PubMed ID

    21476140

    Content Manager
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