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    In vitro inactivation of Chlamydia trachomatis and of a panel of DNA (HSV-2, CMV, adenovirus, BK virus) and RNA (RSV, enterovirus) viruses by the spermicide benzalkonium chloride.

    Authors

    Bélec L, Tevi-Benissan C, Bianchi A, et al. 

    Source

    J Antimicrob Chemother 2000 Nov; 46(5) :685-93.

    Abstract

    Kinetics of inactivation by the detergent spermicide benzalkonium chloride (BZK) of Chlamydia trachomatis and of a panel of DNA viruses [herpes simplex virus hominis type 2 (HSV-2), cytomegalovirus (CMV), adenovirus (ADV) and BK virus (BKV)] and RNA [respiratory syncytial virus (RSV) and enterovirus (ENV)] were established in accordance with a standardized in vitro protocol. After a 5 min incubation, inactivation of >95% of HSV-2 and CMV was obtained at a concentration of 0.0025% (w/v) (25 Ig/L); concentrations as low as 0.0005%, 0.0050% and 0.0125%, induced a 3.0 log10 reduction in infectivity of HSV-2 and CMV, RSV and ADV, respectively. After a 60 min incubation, concentrations of 0.0125% and 0.050% provided a 3.0 log10 reduction in infectivity of ENV and BKV, respectively. These features indicate that sensitivity to BZK was very high (HSV-2 and CMV) or high (RSV) for enveloped viruses, intermediate (ADV) or low (ENV and BKV) for non-enveloped viruses. Furthermore, BZK had marked antichlamydial activity, showing >99% killing after only a 1 min incubation at a concentration of 0.00125%. BZK demonstrates potent in vitro activity against the majority of microorganisms causing sexually transmitted infectious diseases, including those acting as major genital cofactors of human immunodeficiency virus transmission. These attributes qualify BZK as a particularly attractive candidate for microbicide development.

    Mesh

    Adenoviridae
    Anti-Infective Agents, Local
    BK Virus
    Benzalkonium Compounds
    Chlamydia trachomatis
    Cytomegalovirus
    DNA Viruses
    Enterovirus
    Herpesvirus 2, Human
    Humans
    RNA Viruses
    Respiratory Syncytial Viruses
    Sexually Transmitted Diseases, Viral
    Spermatocidal Agents

    Language

    eng

    Pub Type(s)

    Journal Article Research Support, Non-U.S. Gov't

    PubMed ID

    11062186

    Content Manager
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