Awale S, Shrestha SP, Tezuka Y, et al.
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
SourceJ Nat Prod 2005 Jun; 68(6)
A methanolic extract of Nepalese propolis yielded 10 new open-chain neoflavonoids (1-10), a new chalcone (11), and eight previously reported compounds (12-19). Their structures were determined on the basis of spectroscopic data and chemical conversion. The isolated compounds other than 5, 8, and 16 showed dose-dependent inhibitory activities on nitric oxide production in lipopolysaccharide-activated macrophage-like J774.1 cells and were more active than a positive control, N(G)-monomethyl-L-arginine (IC(50), 27.1 microM). The most potent activities, of 6 and 7 (IC(50), 0.5 microM), were greater than another positive control, caffeic acid phenethyl ester (IC(50), 4.8 microM).
MeshCaffeic AcidsFlavonoidsInhibitory Concentration 50LipopolysaccharidesMacrophagesMolecular StructureNepalNitric OxideNuclear Magnetic Resonance, BiomolecularPhenylethyl AlcoholPropolisomega-N-Methylarginine
Journal Article Research Support, Non-U.S. Gov't