MEDLINE Journals

    Cuban neonatal screening of phenylketonuria using an ultramicro-fluorometric test.

    Authors

    González EC, Frómeta A, del Río L, et al. 

    Source

    Clin Chim Acta 2009 Apr; 402(1-2) :129-32.

    Abstract

    BACKGROUND
    Guthrie's bacterial inhibition assay was used in Cuba, since 1983. A decentralized program for the newborn screening of hyperphenylalaninemias started in the year 2000 using an ultramicro-fluorometric test (UMTEST PKU).
    METHODS
    A simple and rapid ultramicro-fluorometric test based on McCaman and Robin's method has been designed, developed and applied for the measurement of Phe in dried blood spots on filter paper.
    RESULTS
    The UMTEST PKU exhibited an acceptable precision and accuracy. Samples of 27528 newborns on filter paper Schleicher & Schuell 903 (S&S 903) from the National neonatal screening program were collected and analyzed, and the mean Phe concentration was 66.5 micromol/l. Our assay showed high Pearson and concordance correlations with 2 commercially available kits. A total of 521923 Cuban newborns were studied from the year 2000 to 2007 using the UMTEST PKU. Elevated blood phenylalanine levels were found in 1764 infants (0.34%) and no false negative were noted. Ten cases were diagnosed with phenylketonuria, all of them with an initial phenylalanine concentration over 360 micromol/l.
    CONCLUSIONS
    The analytical performance characteristics of our assay and its use in the National program have demonstrated its suitability for the neonatal screening of PKU.

    Mesh

    Blood Specimen Collection
    Cuba
    Fluorometry
    Humans
    Infant, Newborn
    Neonatal Screening
    Phenylalanine
    Phenylketonurias
    Sensitivity and Specificity

    Language

    eng

    Pub Type(s)

    Journal Article

    PubMed ID

    19168043

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