MEDLINE Journals

    Evaluation of the effect of acetyl L-carnitine on experimental cisplatin ototoxicity and neurotoxicity.

    Authors

    Gunes D, Kirkim G, Kolatan E, et al. 

    Institution

    Department of Pediatric Oncology, Institute of Oncology, Dokuz Eylul University, Izmir, Turkey. dilek.gunes@deu.edu.tr

    Source

    Chemotherapy 2011; 57(3) :186-94.

    Abstract

    INTRODUCTION
    Cisplatin (CDDP) is an effective and widely used chemotherapeutic agent for pediatric tumors, and ototoxicity is one of the dose-limiting side effects.
    OBJECTIVE
    It was the aim of our study to investigate the effect of acetyl L-carnitine (ALCAR) on experimental CDDP ototoxicity by audiologic tests, histomorphologic, immunohistochemical and ultrastructural examinations and to investigate the apoptotic pathways.
    MATERIALS AND METHODS
    Wistar albino rats (n = 28) were studied. Baseline audiological tests were performed in 4 groups: group 1, control; group 2, ALCAR; group 3, CDDP; group 4, CDDP + ALCAR-administered rats. Control audiological tests were performed on the 3rd day, and then the rats were sacrificed. Ear and brain specimens were examined by transmission electron microscopy, and caspase 3, 8 and 9 activities were investigated.
    RESULTS
    The CDDP-administered rats showed significant auditory brainstem response threshold shifts using all stimuli (clicks, 6-kHz and 8-kHz tone burst) compared with the control groups. The CDDP + ALCAR-administered rats showed significant auditory brainstem response threshold shifts by only click stimuli compared with the control groups. In the brain, spiral ganglion and organ of Corti, ultrastructural damage was prominent in group 3; the number of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling)-positive cells and caspase 3, 8 and 9 immunostaining cells was significantly high in group 3.
    CONCLUSION
    ALCAR improves CDDP-induced auditory impairment, and also antioxidative and antiapoptotic properties of ALCAR on CDDP ototoxicity were supported by the findings.

    Mesh

    Acetylcarnitine
    Animals
    Antineoplastic Agents
    Brain
    Caspase 3
    Caspase 8
    Caspase 9
    Cisplatin
    Ear, Middle
    Evoked Potentials, Auditory, Brain Stem
    Female
    Hair Cells, Auditory
    Hearing Disorders
    Organ of Corti
    Rats
    Rats, Wistar
    Vitamin B Complex

    Language

    eng

    Pub Type(s)

    Evaluation Studies Journal Article

    PubMed ID

    21525749

    Content Manager
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