MEDLINE Journals

    An expanded multilocus sequence typing scheme for propionibacterium acnes: investigation of 'pathogenic', 'commensal' and antibiotic resistant strains.

    Authors

    McDowell A, Barnard E, Nagy I, et al. 

    Institution

    Centre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, Queen's University, Belfast, United Kingdom. a.mcdowell@qub.ac.uk

    Source

    PLoS One 2012; 7(7) :e41480.

    Abstract

    The Gram-positive bacterium Propionibacterium acnes is a member of the normal human skin microbiota and is associated with various infections and clinical conditions. There is tentative evidence to suggest that certain lineages may be associated with disease and others with health. We recently described a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes (http://pubmlst.org/pacnes). We now describe an expanded eight gene version based on six housekeeping genes and two 'putative virulence' genes (eMLST) that provides improved high resolution typing (91eSTs from 285 isolates), and generates phylogenies congruent with those based on whole genome analysis. When compared with the nine gene MLST scheme developed at the University of Bath, UK, and utilised by researchers at Aarhus University, Denmark, the eMLST method offers greater resolution. Using the scheme, we examined 208 isolates from disparate clinical sources, and 77 isolates from healthy skin. Acne was predominately associated with type IA(1) clonal complexes CC1, CC3 and CC4; with eST1 and eST3 lineages being highly represented. In contrast, type IA(2) strains were recovered at a rate similar to type IB and II organisms. Ophthalmic infections were predominately associated with type IA(1) and IA(2) strains, while type IB and II were more frequently recovered from soft tissue and retrieved medical devices. Strains with rRNA mutations conferring resistance to antibiotics used in acne treatment were dominated by eST3, with some evidence for intercontinental spread. In contrast, despite its high association with acne, only a small number of resistant CC1 eSTs were identified. A number of eSTs were only recovered from healthy skin, particularly eSTs representing CC72 (type II) and CC77 (type III). Collectively our data lends support to the view that pathogenic versus truly commensal lineages of P. acnes may exist. This is likely to have important therapeutic and diagnostic implications.

    Mesh

    Acne Vulgaris
    Bacterial Typing Techniques
    Databases, Genetic
    Drug Resistance, Bacterial
    Endophthalmitis
    Evolution, Molecular
    Genes, Bacterial
    Genes, Essential
    Gram-Positive Bacterial Infections
    Humans
    Keratitis
    Linkage Disequilibrium
    Multilocus Sequence Typing
    Mutation
    Phylogeny
    Propionibacterium acnes
    RNA, Bacterial
    RNA, Ribosomal
    Selection, Genetic
    Skin
    Virulence Factors

    Language

    eng

    Pub Type(s)

    Journal Article Research Support, Non-U.S. Gov't

    PubMed ID

    22859988

    Content Manager
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