MEDLINE Journals

    Ultrafiltration and endotoxin removal from dialysis fluids.

    Authors

    Di Felice A, Cappelli G, Facchini F, et al. 

    Institution

    Department of Nephrology, University of Modena, Bellco Laboratories, Bellco Spa, Italy.

    Source

    Kidney Int Suppl 1993 Jun.:S201-4.

    Abstract

    Biocompatibility in hemodialysis is now regarded as a multifactorial problem and dialysate represents a main risk. Pyrogenic fractions mostly coming from gram-negative bacteria easily pass through dialysis membrane, either by backdiffusion or by backfiltration, and induce blood cell activation. To demonstrate the long-term efficiency of a 2 m2 polyamide ultrafilter in producing a pyrogen free solution, we used an experimental circuit ultrafiltering for 240 hours (500 ml/min) a bicarbonate dialysate contaminated (5 to 48 EU/ml) by a Pseudomonas aeruginosa filtrate. The efficiency was monitored by LAL-test and IL-1 PBMC so to detect not only lipid A containing endotoxins but also other cytokines inducing bacterial fractions. At the post-ultrafilter sampling port the LAL-test was < 0.005 to 0.034 EU/ml; IL-1 PBMC was below the detection limit (20 pg/ml) being 27 to 63 pg/ml at the pre-ultrafilter level. Polyamide ultrafiltration represents an efficient system to obtain an endotoxin-free dialysate and a single filter works up to 240 hours.

    Mesh

    Cells, Cultured
    Drug Contamination
    Endotoxins
    Hemodialysis Solutions
    Humans
    Interleukin-1
    Leukocytes, Mononuclear
    Membranes, Artificial
    Molecular Weight
    Pyrogens
    Renal Dialysis
    Ultrafiltration

    Language

    eng

    Pub Type(s)

    Journal Article

    PubMed ID

    8320921

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