Unbound MEDLINE

Neutral proteases in crevicular fluid as an indicator for periodontal treatment intervention.

Abstract

PURPOSE
To longitudinally determine if early therapeutic intervention, based on a positive neutral protease activity (NPA) assay score could effectively arrest the progress of periodontal disease destruction.
MATERIALS AND METHODS
63 periodontal sites which had previously undergone probing attachment loss were identified from among 31 previously treated adult periodontitis patients who were monitored during periodontal maintenance for an average of 3 yrs. Clinical levels of gingival inflammation and attachment levels and NPA assay data were collected at the beginning of each maintenance visit. When a site tested negative with the assay, routine Supportive Periodontal Therapy (SPT) was followed during the same appointment, while sites exhibiting a positive NPA score received more aggressive periodontal treatment.
RESULTS
During the study period, 51 of 63 sites displayed at least one positive NPA score. Our protocol of administering periodontal treatment rendered at the visit showing a positive NPA score revealed that only 1 of the 51 sites lost > or = 1 mm attachment during the study period. The remaining 50 positive assay sites showed an overall gain of > or = 1 mm of probing attachment over the course of the study. 12 of 63 sites consistently tested negative for neutral protease enzyme activity and remained stable, although 9 of these sites exhibited bleeding on probing (BOP) at least once during this study. Initial group mean probing attachment measurements were 5.6 mm for NPA negative and 5.7 mm for NPA positive sites.

Authors

Bader HI, Boyd RL

Institution

Department of Periodontology, Harvard School of Dental Medicine, Boston, Massachusetts, USA.

Source

American journal of dentistry 14:5 2001 Oct pg 314-8

MeSH

Adult
Aged
Analysis of Variance
Anti-Bacterial Agents
Anti-Infective Agents, Local
Biocompatible Materials
Cellulose
Chlorhexidine
Dental Plaque
Dental Scaling
Drug Delivery Systems
Endopeptidases
Female
Follow-Up Studies
Gingival Crevicular Fluid
Gingival Hemorrhage
Gingivitis
Humans
Longitudinal Studies
Male
Middle Aged
Periodontal Attachment Loss
Periodontal Diseases
Periodontitis
Reproducibility of Results
Root Planing
Subgingival Curettage
Tetracycline

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11803997