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Distribution of the CCR5 gene 32-base pair deletion and CCR5 expression in Chinese minorities.

Abstract

China has an ethnically diverse population. Genetic differences may contribute to disparities in the efficiency of HIV transmission. To further characterize this risk, we examined the HIV-related genetic diversity in the predominant Han Chinese and in six minority groups. We searched for the delta32-CCR5 mutation, a common cause of relative HIV resistance in the white population. In addition, CCR5 receptor expression was measured. Blood samples were obtained from adults belonging to the Han, Meng, Zang, Weiwuer, Zhuang, Yi, and Dai ethnic groups. Polymerase chain reaction analysis was performed on genomic DNA samples. Surface expression of CCR5 on peripheral blood mononuclear cells was measured by flow cytometry. One-way ANOVA was used to determine mean statistical differences. Samples from 10 members of each minority were examined. A delta32-CCR5 heterozygote phenotype was detected in one Weiwuer subject, but no mutations were found in the other 69 subjects studied. The mean CCR5 expression of cells harvested from the Dai minority was greater than that of cells from all other minorities studied, for both CD3+CCR5+ and CD4+CCR5+ sets (p < .01, one-way ANOVA). The delta32-CCR5 mutation seems to be rare in most Han Chinese and the minority populations studied. CCR5 expression appears to be greater in the Dai minority than in the other minorities investigated. The mechanism for this increased expression requires further study.

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  • Publisher Full Text
  • Authors

    Feng T, Ni A, Yang G, Galvin SR, Hoffman IF, Cohen MS

    Institution

    Department of Clinical Immunology, Peking Union Medical College Hospital, Beijing, People's Republic of China.

    Source

    Journal of acquired immune deficiency syndromes (1999) 32:2 2003 Feb 1 pg 131-4

    MeSH

    Adult
    Antigens, CD3
    Antigens, CD4
    Asian Continental Ancestry Group
    Base Pairing
    China
    Flow Cytometry
    Gene Deletion
    Gene Expression
    Genetic Predisposition to Disease
    Genetics, Population
    HIV Infections
    Heterozygote
    Humans
    Leukocytes, Mononuclear
    Minority Groups
    Mutation
    Polymerase Chain Reaction
    Receptors, CCR5
    Risk Factors

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    12571521