Abstract

Cis-diaminedichloroplatinum (cisplatin) has therapeutic efficacy against advanced bladder cancer. However, the response is often limited due to appearance of drug-resistant tumor cells. Recently, we established a cisplatin-resistant human bladder cancer cell line, T24/R2, from T24 human bladder cancer cell line, which shows 18-fold resistance to cisplatin by the stepwise exposure of increasing concentrations of cisplatin. A light microscopic (LM) and transmission electron microscopic (EM) examination was performed to investigate the morphological and ultrastructural changes during induction of drug resistance. In LM, the cytoplasm of T24R2 cells showed plumper pattern than that of T24 parent cells. In EM, the chromatin pattern of T24R2 cells was finely dispersed compared to T24 cells, which were coarse and aggregated. The mitochondrial volume, rough endoplasmic reticulum, polyribosomes and ribosomes were moderately increased in T24R2 cells. The cell membrane showed ruffled border and great amount of double membrane vesicles and pinocytic vesicles were observed in the cell surface of T24R2 cells, which were seldom observed in T24 cells. With these findings, we concluded that human bladder cancer cells underwent morphological and ultrastructural changes during acquiring of resistance to cisplatin. We could suggest that these changes might be involved in the drug resistance mechanism in human bladder cancer cells.

Links

Publisher Full Text

Authors

Yoon SJ, Park I, Kwak C, Lee E

Institution

Department of Urology, Gachon Medical School, Incheon 405-760, Korea.

Source

Oncology reports 10:5 pg 1363-7

MeSH

Antineoplastic Agents
Cell Line, Tumor
Cell Membrane
Chromatin
Cisplatin
Cytoplasm
Drug Resistance, Neoplasm
Endoplasmic Reticulum, Rough
Humans
Inhibitory Concentration 50
Microscopy, Electron
Mitochondria
Ribosomes
Urinary Bladder Neoplasms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12883708