Unbound MEDLINE

Cytotoxic and apoptotic effects on hepatocytes of secondary metabolites obtained from lichens.

Abstract

There are a large number of species of Antarctic lichens, and several studies describing the secondary metabolites present in these lichens, as well as the advances in understanding the chemistry of these metabolites, have been reported. In addition, some derivatives displaying interesting antibacterial effects have been described. The cytotoxic and apoptotic effects of 15 secondary metabolites (depsides, depsidones and usnic acid) obtained from Continental (Chilean) and Antarctic lichens were evaluated in primary cultures of rat hepatocytes. Intracellular lactate dehydrogenase release, caspase 3 activation and DNA fragmentation were measured. In this study, we have evaluated a set of markers associated with pivotal steps in the execution phase of apoptosis, in order to detect compounds with apoptotic effects on hepatocytes before significant necrosis takes place. Flow cytometric analysis of DNA fragmentation revealed an increase in apoptotic nuclei with sub-diploid DNA content after the exposure of hepatocytes to sub-cytotoxic concentrations of the compounds. Among these, salazinic acid, stictic acid and psoromic acid displayed significant apoptotic activities.

Authors

Correché ER, Enriz RD, Piovano M, Garbarino J, Gómez-Lechón MJ

Institution

Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 915, 5700 San Luis, Argentina.

Source

Alternatives to laboratory animals : ATLA 32:6 2004 Dec pg 605-15

MeSH

Animals
Antarctic Regions
Apoptosis
Benzofurans
Benzoic Acids
Carboxylic Acids
Caspase 3
Caspases
Depsides
Enzyme Activation
Flow Cytometry
Hepatocytes
Hydroxybenzoic Acids
Lactones
Lichens
Male
Rats
Rats, Sprague-Dawley

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15757498