Abstract

The fluorochrome, p-hydrazinoacridine (HA), is used to stain selectively natural macromolecular aldehydes as occur in elastic tissue as well as periodic acid (oxidized) or hydrochloric acid (hydrolyzed) engendered polyaldehydes (e.g., respectively of glycogen, mucosubstances and deoxyribonucleic acid). The reaction is independent of SO2 required in Schiff-type reactions. The mechanism of the staining reaction is presumably via the formation of fluorescent hydrazones from aldehyde condensation with HA. Aldehyde blocking agents, i.e., thiosemicarbazide and sodium borohydride, block the staining reactions with HA. In view of the controversy surrounding the mechanism of Schiff-type reactions and the lack of an adequate explanation for the affinity of miscellaneous fluorochromes for elastic tissue, p-hydrazinoacridine appears to be an ideal reagent, combining a simple staining procedure, excellent histological results and a straightforward mechanism for its selectivity.

Links

Aggregator Full Text

Authors

Weinblatt FM, Shannon WA, Seligman AM

Source

Histochemistry 41:4 1975 pg 353-9

MeSH

Acridines
Aldehydes
Animals
Elastic Tissue
Fluorescence
Histocytochemistry
Hydrazines
Hydrazones
Hydrochloric Acid
Macromolecular Substances
Periodic Acid
Rats
Schiff Bases
Sulfur Dioxide

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

166965