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Expression of inflammation-related genes in endothelial cells is not directly affected by microparticles from preeclamptic patients.

Abstract

BACKGROUND
Inflammation and endothelial dysfunction are prominent in preeclampsia. Microparticles (MPs) may link these processes, as MPs induce the production of pro-inflammatory cytokines by endothelial cells and cause endothelial dysfunction.
AIM
To study changes in expression of inflammation-related genes in human endothelial cells in response to MPs from preeclamptic patients.
METHODS
Human umbilical vein endothelial cells (HUVECs) were incubated for various time intervals in the absence or presence of isolated MP fractions from preeclamptic patients (n = 3), normotensive pregnant women (n = 3), non-pregnant controls (n = 3), and interleukin (IL)-1alpha as a positive control. Total RNA was isolated and used for multiplex ligation-dependent probe amplification (MLPA) and real-time polymerase chain reaction (PCR).
RESULTS
IL-1alpha enhanced the expression of IL-1alpha, IL-2, IL-6, and IL-8; nuclear factor of kappa light chain enhancer in B-cells (NFkappaB)-1, NFkappaB-2, and NFkappaB-inhibitor; cyclin-dependent kinase inhibitor and monocyte chemotactic protein-1; and transiently increased tissue factor expression. RNA expression of inflammation-related genes and genes encoding adhesion receptors, however, were unaffected by any of the MP fractions tested.
CONCLUSION
MLPA is a suitable assay to test the inflammatory status of endothelial cells, because incubation with IL-1alpha triggered substantial changes in RNA expression in endothelial cells. Taken together, it seems unlikely that MPs from preeclamptic patients induce endothelial dysfunction by directly affecting the expression of inflammation-related genes in these cells.

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  • Publisher Full Text

    • Authors

    Lok CA, Böing AN, Reitsma PH, van der Post JA, van Bavel E, Boer K, Sturk A, Nieuwland R

    Institution

    Department of Obstetrics, Academic Medical Center, Amsterdam, The Netherlands. c.a.lok@amc.uva.nl

    Source

    The Journal of laboratory and clinical medicine 147:6 2006 Jun pg 310-20

    MeSH

    Adult
    Chemokine CCL2
    Cytokines
    Endothelial Cells
    Female
    Gene Expression Regulation
    Humans
    Interleukin-1
    Interleukin-2
    Interleukin-6
    Interleukin-8
    NF-kappa B
    Particle Size
    Pre-Eclampsia
    Pregnancy
    RNA, Messenger
    Reverse Transcriptase Polymerase Chain Reaction
    Umbilical Veins

    Pub Type(s)

    In Vitro
    Journal Article

    Language

    eng

    PubMed ID

    16750669