Abstract

Cancer targeting is crucial for cancer detection, therapy and targeted drug delivery. A dendrimer-peptide conjugate has been synthesized based on poly(amidoamine) dendrimer generation 5 (PAMAM G5) as a platform and a luteinizing hormone-releasing hormone (LHRH) peptide as a targeting moiety. The synthesized conjugate was fully characterized using nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Further stability experiments showed that the synthesized conjugate was stable after 72-h incubation in phosphate-buffered saline (PBS) buffer (pH 7.4) at 37 degrees C. The synthesized conjugate may find applications in biomedical targeting, gene delivery and imaging.

Links

Publisher Full Text

Authors

Bi X, Shi X, Baker JR

Institution

Department of Physical Sciences, Charleston Southern University, Charleston, SC 29406, USA. xbi@csuniv.edu

Source

Journal of biomaterials science. Polymer edition 19:1 2008 pg 131-42

MeSH

Biocompatible Materials
Chromatography, High Pressure Liquid
Dendrimers
Gonadotropin-Releasing Hormone
Humans
Magnetic Resonance Spectroscopy
Models, Chemical
Molecular Structure
Molecular Weight
Polyamines
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18177559