Synthesis, characterization and stability of a luteinizing hormone-releasing hormone (LHRH)-functionalized poly(amidoamine) dendrimer conjugate.
Cancer targeting is crucial for cancer detection, therapy and targeted drug delivery. A dendrimer-peptide conjugate has been synthesized based on poly(amidoamine) dendrimer generation 5 (PAMAM G5) as a platform and a luteinizing hormone-releasing hormone (LHRH) peptide as a targeting moiety. The synthesized conjugate was fully characterized using nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Further stability experiments showed that the synthesized conjugate was stable after 72-h incubation in phosphate-buffered saline (PBS) buffer (pH 7.4) at 37 degrees C. The synthesized conjugate may find applications in biomedical targeting, gene delivery and imaging.
Department of Physical Sciences, Charleston Southern University, Charleston, SC 29406, USA. email@example.com
SourceJournal of biomaterials science. Polymer edition 19:1 2008 pg 131-42
Chromatography, High Pressure Liquid
Magnetic Resonance Spectroscopy
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pub Type(s)Journal Article
Research Support, N.I.H., Extramural