Unbound MEDLINE

Modulatory effects of N-acetylcysteine on hyperoxaluric manifestations in rat kidney.

Abstract

Hyperoxaluria is a condition where excessive oxalate is present in the urine. Many reports have documented free radical generation followed by hyperoxaluria as a consequence of which calcium oxalate deposition occurs in the kidney tissue. The present invivo study was designed to investigate the potential of N-acetylcysteine in modulating hyperoxaluric manifestation induced by sodium oxalate in the rat kidneys. Male wistar rats in one group were administered single dose of sodium oxalate (70mg/kg body weight) intraperitoneally to induce hyperoxaluric conditions and in the other group, rats were injected N-acetylcysteine (NAC) (200mg/kg body weight) intraperitoneally, half an hour after sodium oxalate dose. The treatment is for a period of 24h. N-acetylcysteine significantly reduced hyperoxaluria caused oxidative stress by reducing lipid peroxidation, restoring antioxidant enzymes activity in kidney tissue, followed by reduction in impairment of renal functioning. In addition, NAC administration reduced the number of calcium oxalate monohydrate (COM) crystals in the urine as observed under polarization microscope. Histological analysis depicted that NAC treatment decreased renal epithelial damage, inflammation and restored normal glomeruli morphology. Thus, it shows that use of an extraneous antioxidant may prove beneficial for combating the conditions of oxidative stress produced by hyperoxaluria.

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  • Publisher Full Text
  • Authors

    Bijarnia RK, Kaur T, Aggarwal K, Singla SK, Tandon C

    Institution

    Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan 173 215, India.

    Source

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 46:6 2008 Jun pg 2274-8

    MeSH

    Acetylcysteine
    Animals
    Antioxidants
    Catalase
    Creatinine
    Free Radical Scavengers
    Hyperoxaluria
    Kidney
    Kidney Calculi
    Male
    Oxalic Acid
    Oxidative Stress
    Phospholipids
    Rats
    Rats, Wistar
    Superoxide Dismutase
    Urea
    Urodynamics

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    18423961