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[DNA polymorphism detection of Cannabis using amplified fragment length polymorphism].

Abstract

OBJECTIVE
To screen the AFLP primers with good diversity to distinguish various species of Cannabis.
METHODS
The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.
RESULTS
A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.
CONCLUSION
AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.

Authors

Guo J, Pei L, Peng JX, Zhai H, Zhang GQ, Jie Q, Tu Z

Source

Fa yi xue za zhi 24:5 2008 Oct pg 330-2

MeSH

Amplified Fragment Length Polymorphism Analysis
Cannabis
DNA, Plant
Forensic Genetics
Genetic Variation
Polymorphism, Genetic

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18979914