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[DNA polymorphism detection of Cannabis using amplified fragment length polymorphism].
OBJECTIVETo screen the AFLP primers with good diversity to distinguish various species of Cannabis.
METHODSThe AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.
RESULTSA total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.
CONCLUSIONAFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
Fa yi xue za zhi 24:5 2008 Oct pg 330-2
MeSHAmplified Fragment Length Polymorphism Analysis
Pub Type(s)English Abstract
Research Support, Non-U.S. Gov't