Unbound MEDLINE

Ent-16β,17-dihydroxy-kauran-19-oic acid, a kaurane diterpene acid from Siegesbeckia pubescens, presents antiplatelet and antithrombotic effects in rats.

Abstract

The antiplatelet and antithrombotic effects of ent-16β,17-dihydroxy-kauran-19-oic acid (DDKA) isolated from Siegesbeckia pubescens were investigated with different methods both in vitro and in vivo. We tested the antithrombotic activity of DDKA in arterio-venous shunt model. The effects of DDKA on adenosine diphosphate (ADP)-, Thrombin-, Arachidonic acid-induced rat platelets aggregation were tested in vitro. We also assessed its bleeding side effect by measuring coagulation parameters after intravenous administration for 5 days and investigated the potential mechanisms underlying such activities. In vivo, DDKA significantly reduced thrombus weight in the model of arterio-venous shunt. Meanwhile, DDKA increased plasma cAMP level determined by radioimmunoassay in the same model. Notably, DDKA prolonged PT and APTT in rats after intravenous administration DDKA for successive 5 days. In vitro, pretreatment with DDKA on washed rat platelets significantly inhibited various agonists stimulated platelet aggregation and caused an increase in cAMP level in platelets activated by ADP. These findings support our hypothesis that DDKA possesses antiplatelet and antithrombotic activities. The mechanisms underlying such activities may involve the anticoagulatory effect and cAMP induction.

Links

  • Publisher Full Text

    • Authors

    Wang JP, Xu HX, Wu YX, Ye YJ, Ruan JL, Xiong CM, Cai YL

    Institution

    Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong Road, Wuhan 430030, PR China.

    Source

    Phytomedicine : international journal of phytotherapy and phytopharmacology 18:10 2011 Jul 15 pg 873-8

    MeSH

    6-Ketoprostaglandin F1 alpha
    Adenosine Diphosphate
    Animals
    Asteraceae
    Coronary Thrombosis
    Cyclic AMP
    Diterpenes, Kaurane
    Epoprostenol
    Female
    Fibrinolytic Agents
    Hemorrhage
    Male
    Mice
    Mice, Inbred ICR
    Platelet Aggregation
    Platelet Aggregation Inhibitors
    Rats
    Rats, Wistar
    Thrombin
    Thromboxane A2
    Thyroglobulin

    Pub Type(s)

    In Vitro
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21377851