Abstract

The objective of the present study was to examine the phylogenetic position of Setaria cervi based on sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. A fragment of the cox1 gene from two morphologically identified S. cervi collected from red deer (Cervus elaphus) from Italy were amplified, sequenced, and compared with corresponding sequences of other filarioid nematode species. Phylogenetic studies using Bayesian analysis revealed S. cervi as monophyletic with other Setaria species, confirming S. cervi as a member of the Setaria genus. S. cervi appeared to be sister species to Setaria labiatopapillosa and Setaria digitata. Setaria tundra and Setaria equina, the other two Setaria species presented in the Italian fauna, formed a sister group to the clade consisting of S. cervi, S. labiatopapillosa, and S. digitata. In addition to phylogenetic clarification, our study is the first molecular identification of S. cervi, which may be useful for further molecular identification and differentiation of this filarial worm from other filarioid nematode species, especially in the earlier developmental stages of its life cycle.

Links

Publisher Full Text

Authors

Alasaad S, Pascucci I, Jowers MJ, Soriguer RC, Zhu XQ, Rossi L

Institution

Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Via Leonardo da Vinci 44, 10095, Grugliasco, Italy. samer@ebd.csic.es

Source

Parasitology research 110:1 2012 Jan pg 281-5

MeSH

Animals
Cluster Analysis
Cyclooxygenase 1
DNA, Mitochondrial
Deer
Female
Filarioidea
Italy
Male
Mitochondrial Proteins
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21695569