Abstract

Tissue engineering technique seems to be the best strategy for treating the urethra disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) "neourethra" tissue for repairing the urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved urethra in group A showed fibrosis and inflammation during 6 months. Simple epithelial layer regenerated in group B while there was no evidence of CSMCs growing into grafts during study period. Stratified epithelial layer and organized muscle fiber bundles were evident after 6 months in group C. Our study suggested that lingual keratinocytes and CSMCs could be used as a source of seed cells for urethral tissue engineering. Using the dynamic-static seeding method, 3D urethra could be constructed in vivo.

Links

Publisher Full Text

Authors

Feng C, Xu YM, Fu Q, Zhu WD, Cui L

Institution

Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, People's Republic of China.

Source

Tissue engineering. Part A 17:23-24 2011 Dec pg 3011-9

MeSH

Animals
Cell Shape
Cells, Cultured
Immunohistochemistry
Keratinocytes
Male
Mechanical Phenomena
Myocytes, Smooth Muscle
Penis
Rabbits
Reconstructive Surgical Procedures
Sus scrofa
Tissue Scaffolds
Tongue
Urethra
Urologic Surgical Procedures

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21736450