Abstract

BACKGROUND
The efficiency of bioethanol production from wheat biomass is related to the quality of end products as well as to safety criteria of co-products such as distiller's dried grains with solubles (DDGS). The inclusion of a new biocatalyst for non-starch polysaccharide degradation in fermentation processes could be one of the solutions. The objective of this study was to evaluate the influence of β-xylanases in combination with traditional amylolytic enzymes on the efficiency of bioethanol production and DON detoxification during fermentation of Fusarium-contaminated wheat biomass with high concentration of deoxynivalenol (DON; 3.95 mg kg(-1)).
RESULTS
The results showed that the negative effect of Fusarium spp. on yield and quality of bioethanol could be eliminated by the application of Trichoderma reesei xylanase in combination with amylolytic enzymes. This technological solution allowed to increase the concentration of ethanol in the fermented wort by 35.3% and to improve the quality of bioethanol by decreasing the concentrations of methanol, methyl acetate, isoamyl and isobutyl alcohols. Mass balance calculations showed that DDGS was the main source of DON contamination, comprising 74% of toxin found in wheat biomass. By using new enzyme combination for wheat biomass saccharification, a higher level of detoxification (41%) of DON was achieved during the fermentation process.
CONCLUSION
The addition of Trichoderma reesei xylanase played a positive role in bioethanol production from Fusarium-contaminated wheat biomass, indicating that the yeast-growing medium was enriched during the enzymatic treatment.

Links

Publisher Full Text

Authors

Juodeikiene G, Basinskiene L, Vidmantiene D, Makaravicius T, Bartkiene E

Institution

Kaunas University of Technology, Radvilenu pl. 19, LT-50254 Kaunas, Lithuania. grazina.juodeikiene@ktu.lt

Source

Journal of the science of food and agriculture 92:1 2012 Jan 15 pg 84-91

MeSH

Acetates
Alcohols
Amylases
Biofuels
Biomass
Cereals
Distillation
Endo-1,4-beta Xylanases
Ethanol
Fermentation
Fusarium
Polysaccharides
Trichoderma
Trichothecenes
Triticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21744360