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The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Abstract

BACKGROUND
Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.
MATERIAL/METHODS
We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.
RESULTS
The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).
CONCLUSIONS
The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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  • Authors

    Szeliga J, Klodzinska E, Jackowski M, Buszewski B

    Source

    Medical science monitor : international medical journal of experimental and clinical research 17:10 2011 Oct pg MT91-6

    MeSH

    Boric Acids
    Edetic Acid
    Electrophoresis, Capillary
    Escherichia coli Infections
    Humans
    Sensitivity and Specificity
    Surgical Wound Infection
    Tromethamine

    Pub Type(s)

    Comparative Study
    Evaluation Studies
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21959622