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Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity.

Abstract

Dengue viruses (DENV; family Flaviviridae, genus Flavivirus) are transmitted by Aedes aegypti mosquitoes and can cause dengue fever (DF), a relatively benign disease, or more severe dengue haemorrhagic fever (DHF). Arthropod saliva contains proteins delivered into the bite wound that can modulate the host haemostatic and immune responses to facilitate the intake of a blood meal. The potential effects on DENV infection of previous exposure to Ae. aegypti salivary proteins have not been investigated. We collected Ae. aegypti saliva, concentrated the proteins and fractionated them by nondenaturing polyacrylamide gel electrophoresis (PAGE). By the use of immunoblots, we analysed reactivity with the mosquito salivary proteins (MSP) of sera from 96 Thai children diagnosed with secondary DENV infections leading either to DF or DHF, or with no DENV infection, and found that different proportions of each patient group had serum antibodies reactive to specific Ae. aegypti salivary proteins. Our results suggest that prior exposure to MSP might play a role in the outcome of DENV infection in humans.

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  • Authors

    Machain-Williams C, Mammen MP, Zeidner NS, Beaty BJ, Prenni JE, Nisalak A, Blair CD

    Source

    Parasite immunology 34:1 2012 Jan pg 15-22

    MeSH

    Adolescent
    Adult
    Aedes
    Animals
    Child
    Child, Preschool
    Dengue
    Disease Vectors
    Electrophoresis, Polyacrylamide Gel
    Female
    Humans
    Immunoblotting
    Insect Proteins
    Male
    Salivary Proteins and Peptides
    Statistics as Topic
    Thailand
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    21995849