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Development of surface chemistry for surface plasmon resonance based sensors for the detection of proteins and DNA molecules.

Abstract

The immobilisation of biological recognition elements onto a sensor chip surface is a crucial step for the construction of biosensors. While some of the optical biosensors utilise silicon dioxide as the sensor surface, most of the biosensor surfaces are coated with metals for transduction of the signal. Biological recognition elements such as proteins can be adsorbed spontaneously on metal or silicon dioxide substrates but this may denature the molecule and can result in either activity reduction or loss. Self assembled monolayers (SAMs) provide an effective method to protect the biological recognition elements from the sensor surface, thereby providing ligand immobilisation that enables the repeated binding and regeneration cycles to be performed without losing the immobilised ligand, as well as additionally helping to minimise non-specific adsorption. Therefore, in this study different surface chemistries were constructed on SPR sensor chips to investigate protein and DNA immobilisation on Au surfaces. A cysteamine surface and 1%, 10% and 100% mercaptoundecanoic acid (MUDA) coatings with or without dendrimer modification were utilised to construct the various sensor surfaces used in this investigation. A higher response was obtained for NeutrAvidin immobilisation on dendrimer modified surfaces compared to MUDA and cysteamine layers, however, protein or DNA capture responses on the immobilised NeutrAvidin did not show a similar higher response when dendrimer modified surfaces were used.

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  • Publisher Full Text
  • Authors

    Altintas Z, Uludag Y, Gurbuz Y, Tothill I

    Institution

    Cranfield University, Cranfield Health, Cranfield, Bedfordshire, UK.

    Source

    Analytica chimica acta 712: 2012 Jan 27 pg 138-44

    MeSH

    Biosensing Techniques
    Cysteamine
    DNA
    Dendrimers
    Gold
    Nucleic Acid Hybridization
    Proteins
    Surface Plasmon Resonance
    Surface Properties

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22177077