Unbound MEDLINE

Characterization of the ELPhiS prophage from Salmonella enterica serovar Enteritidis strain LK5.

Abstract

Phages are a primary driving force behind the evolution of bacterial pathogens by transferring a variety of virulence genes into their hosts. Similar to other bacterial genomes, the Salmonella enterica serovar Enteritidis LK5 genome contains several regions that are homologous to phages. Although genomic analysis demonstrated the presence of prophages, it was unable to confirm which phage elements within the genome were viable. Genetic markers were used to tag one of the prophages in the genome to allow monitoring of phage induction. Commonly used laboratory strains of Salmonella were resistant to phage infection, and therefore a rapid screen was developed to identify susceptible hosts. This approach showed that a genetically tagged prophage, ELPhiS (Enteritidis lysogenic phage S), was capable of infecting Salmonella serovars that are diverse in host range and virulence and has the potential to laterally transfer genes between these serovars via lysogenic conversion. The rapid screen approach is adaptable to any system with a large collection of isolates and may be used to test the viability of prophages found by sequencing the genomes of various bacterial pathogens.

Links

  • PMC Free PDF
  • Publisher Full Text
  • Authors

    Hanna LF, Matthews TD, Dinsdale EA, Hasty D, Edwards RA

    Institution

    Molecular Sciences Department, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.

    Source

    Applied and environmental microbiology 78:6 2012 Mar pg 1785-93

    MeSH

    DNA, Viral
    Gene Order
    Gene Transfer, Horizontal
    Genes, Viral
    Host Specificity
    Lysogeny
    Molecular Sequence Data
    Prophages
    Salmonella enteritidis
    Sequence Analysis, DNA
    Virulence
    Virus Activation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    22247173