Abstract

INTRODUCTION
Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement.
METHODS
A coil spring was placed in CCR2-deficient (CCR2(-/-)), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction.
RESULTS
Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2(-/-) and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2(-/-) than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups.
CONCLUSIONS
These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.

Links

Publisher Full Text

Authors

Taddei SR, Andrade I, Queiroz-Junior CM, Garlet TP, Garlet GP, Cunha Fde Q, Teixeira MM, da Silva TA

Institution

Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

Source

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 141:2 2012 Feb pg 153-60

MeSH

Acid Phosphatase
Animals
Biological Markers
Bone Remodeling
Bone Resorption
Cell Count
Chemokine CCL2
Chemotaxis, Leukocyte
Collagen Type I
Isoenzymes
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Mice, Knockout
Orthodontic Wires
Osteoblasts
Osteocalcin
Osteoclasts
Osteoprotegerin
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Receptors, CCR2
Tooth Movement

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22284282