Unbound MEDLINE

Role of CCR2 in orthodontic tooth movement.

Abstract

INTRODUCTION
Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement.
METHODS
A coil spring was placed in CCR2-deficient (CCR2(-/-)), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction.
RESULTS
Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2(-/-) and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2(-/-) than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups.
CONCLUSIONS
These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.

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  • Publisher Full Text
  • Authors

    Taddei SR, Andrade I, Queiroz-Junior CM, Garlet TP, Garlet GP, Cunha Fde Q, Teixeira MM, da Silva TA

    Source

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 141:2 2012 Feb pg 153-60

    MeSH

    Acid Phosphatase
    Animals
    Biological Markers
    Bone Remodeling
    Bone Resorption
    Cell Count
    Chemokine CCL2
    Chemotaxis, Leukocyte
    Collagen Type I
    Isoenzymes
    Mice
    Mice, Inbred BALB C
    Mice, Inbred Strains
    Mice, Knockout
    Orthodontic Wires
    Osteoblasts
    Osteocalcin
    Osteoclasts
    Osteoprotegerin
    RANK Ligand
    Receptor Activator of Nuclear Factor-kappa B
    Receptors, CCR2
    Tooth Movement

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22284282