Unbound MEDLINE

Role of CCR2 in orthodontic tooth movement.

Abstract

INTRODUCTION
Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement.
METHODS
A coil spring was placed in CCR2-deficient (CCR2(-/-)), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction.
RESULTS
Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2(-/-) and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2(-/-) than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups.
CONCLUSIONS
These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.

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  • Publisher Full Text
  • Authors

    Taddei SR, Andrade I, Queiroz-Junior CM, Garlet TP, Garlet GP, Cunha Fde Q, Teixeira MM, da Silva TA

    Institution

    Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

    Source

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 141:2 2012 Feb pg 153-60

    MeSH

    Acid Phosphatase
    Animals
    Biological Markers
    Bone Remodeling
    Bone Resorption
    Cell Count
    Chemokine CCL2
    Chemotaxis, Leukocyte
    Collagen Type I
    Isoenzymes
    Mice
    Mice, Inbred BALB C
    Mice, Inbred Strains
    Mice, Knockout
    Orthodontic Wires
    Osteoblasts
    Osteocalcin
    Osteoclasts
    Osteoprotegerin
    RANK Ligand
    Receptor Activator of Nuclear Factor-kappa B
    Receptors, CCR2
    Tooth Movement

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22284282