Unbound MEDLINE

Evaluation of VIDAS Salmonella (SLM) easy Salmonella method for the detection of Salmonella in a variety of foods: collaborative study.

Abstract

The VIDAS Salmonella (SLM) Easy Salmonella method is a specific enzyme-linked fluorescent immunoassay performed in the automated VIDAS instrument. The VIDAS Easy Salmonella method is a simple 2-step enrichment procedure, using pre-enrichment followed by selective enrichment in a newly formulated broth, SX2 broth. This new method was compared in a multilaboratory collaborative study to the U.S. Food and Drug Administration's Bacteriological Analytical Manual, Chapter 5 method for five food matrixes (liquid egg, vanilla ice cream, spinach, raw shrimp, and peanut butter) and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook 4.04 method for deli turkey. Each food type was artificially contaminated with Salmonella at three inoculation levels. A total of 15 laboratories representing government, academia, and industry, throughout the United States, participated. In this study, 1583 samples were analyzed, of which 792 were paired replicates and 791 were unpaired replicates. Of the 792 paired replicates, 285 were positive by both the VIDAS and reference methods. Of the 791 unpaired replicates, 341 were positive by the VIDAS method and 325 were positive by the cultural reference method. A Chi-square analysis of each of the six food types was performed at the three inoculation levels tested. For all foods evaluated, the VIDAS Easy SLM method demonstrated results comparable to those of the reference methods for the detection of Salmonella.

Links

  • Publisher Full Text
  • Authors

    Crowley E, Bird P, Fisher K, Goetz K, Benzinger MJ, Agin J, Goins D, Johnson RL

    Institution

    Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA.

    Source

    Journal of AOAC International 94:6 pg 1821-34

    MeSH

    Animals
    Arachis hypogaea
    Cooperative Behavior
    Crustacea
    Eggs
    Enzyme-Linked Immunosorbent Assay
    Fluorescence
    Food Microbiology
    Humans
    Ice Cream
    Laboratories
    Poultry Products
    Reproducibility of Results
    Salmonella
    Sensitivity and Specificity
    Spinacia oleracea
    United States
    United States Food and Drug Administration

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22320090