Abstract

OBJECTIVE
To investigate the expression of the implantation factors leukemia inhibitory factor (LIF), interleukin-11 (IL-11), and IL-6ST/gp130 in the endometrium to examine the relationship between loss of implantation and abnormal uterine cavity.
DESIGN
Case-control study.
SETTING
Department of Obstetrics and Gynecology, Tokyo Medical University.
PATIENT(S)
Subjects comprised 41 patients in the abnormal uterine cavity group who underwent resection of a uterine submucosal myoma or an endometrial polyp by transcervical resectoscopy (TCR) and 18 patients in the control group who underwent laparoscopic surgery.
INTERVENTION(S)
In the abnormal uterine cavity group, endometrial tissue specimens were obtained before resection under hysteroscopy. In the control group, endometrial tissue specimens were obtained by curettage at the time of laparoscopic surgery.
MAIN OUTCOME MEASUREMENT(S)
We divided the patients into four groups according to menstrual cycle and measured the endometrial expression of LIF, IL-11, and IL-6ST/gp130 with the use of quantitative real-time reverse-transcription polymerase chain reaction.
RESULT(S)
Significant increases in LIF and IL-11 mRNA expression were recognized during the midsecretory phase of the menstrual cycle in the control group. However, no difference in IL-6ST/gp130 mRNA expression was observed in any phase during the menstrual cycle in either group. In terms of LIF and IL-11 mRNA expression at the midsecretory phase, the abnormal uterine cavity group showed a significantly decreased LIF mRNA expression compared with the control group.
CONCLUSION(S)
LIF mRNA expression was significantly decreased in abnormal uterine cavities during the midsecretory phase, indicating that endometrial cavity defects are a possible cause of poor reproductive outcomes.

Links

Publisher Full Text

Authors

Hasegawa E, Ito H, Hasegawa F, Hatano K, Kazuka M, Usuda S, Isaka K

Institution

Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, Japan.

Source

Fertility and sterility 97:4 2012 Apr pg 953-8

MeSH

Adult
Case-Control Studies
Cytokine Receptor gp130
Embryo Implantation
Endometrium
Female
Humans
Hysteroscopy
Interleukin-11
Japan
Leiomyoma
Leukemia Inhibitory Factor
Menstrual Cycle
Polyps
RNA, Messenger
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Uterine Neoplasms
Uterus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22341639