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Structural insights into the biogenesis and biofilm formation by the Escherichia coli common pilus.

Abstract

Bacteria have evolved a variety of mechanisms for developing community-based biofilms. These bacterial aggregates are of clinical importance, as they are a major source of recurrent disease. Bacterial surface fibers (pili) permit adherence to biotic and abiotic substrates, often in a highly specific manner. The Escherichia coli common pilus (ECP) represents a remarkable family of extracellular fibers that are associated with both disease-causing and commensal strains. ECP plays a dual role in early-stage biofilm development and host cell recognition. Despite being the most common fimbrial structure, relatively little is known regarding its biogenesis, architecture, and function. Here we report atomic-resolution insight into the biogenesis and architecture of ECP. We also derive a structural model for entwined ECP fibers that not only illuminates interbacteria communication during biofilm formation but also provides a useful foundation for the design of novel nanofibers.

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  • Authors

    Garnett JA, Martínez-Santos VI, Saldaña Z, Pape T, Hawthorne W, Chan J, Simpson PJ, Cota E, Puente JL, Girón JA, Matthews S

    Institution

    Centre for Structural Biology, Department of Biological Sciences, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom.

    Source

    Proceedings of the National Academy of Sciences of the United States of America 109:10 2012 Mar 6 pg 3950-5

    MeSH

    Adhesins, Bacterial
    Bacterial Physiological Phenomena
    Biofilms
    Crystallography, X-Ray
    Escherichia coli
    Escherichia coli Proteins
    Fimbriae Proteins
    Fimbriae, Bacterial
    Genetic Variation
    Microscopy, Electron
    Models, Genetic
    Models, Molecular
    Molecular Chaperones
    Molecular Conformation
    Nanotechnology

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22355107