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Differential effects of energy balance on experimentally-induced colitis.

Abstract

AIM
To characterize the influence of diet-induced changes in body fat on colitis severity in SMAD3-/- mice.
METHODS
SMAD3-/- mice (6-8 wk of age) were randomly assigned to receive a calorie restricted (30% of control; CR), control (CON), or high fat (HF) diet for 20 wk and were gavaged with sterile broth or with Helicobacter hepaticus (H. hepaticus) to induce colitis. Four weeks after infection, mice were sacrificed and the cecum and colons were processed for histological evaluation.
RESULTS
Dietary treatment significantly influenced body composition prior to infection (P < 0.05), with CR mice having less (14% ± 2%) and HF-fed mice more body fat (32% ± 7%) compared to controls (22% ± 4%). Differences in body composition were associated with alterations in plasma levels of leptin (HF > CON > CR) and adiponectin (CON > HF ≥ CR) (P < 0.05). There were no significant differences in colitis scores between CON and HF-fed mice 4 wk post-infection. Consistent with this, differences in proliferation and inflammation markers (COX-2, iNOS), and infiltrating cell types (CD3+ T lymphocytes, macrophages) were not observed. Unexpectedly, only 40% of CR mice survived infection with H. hepaticus, with mortality observed as early as 1 wk following induction of colitis.
CONCLUSION
Increased adiposity does not influence colitis severity in SMAD3-/- mice. Importantly, caloric restriction negatively impacts survival following pathogen challenge, potentially due to an impaired immune response.

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  • Authors

    McCaskey SJ, Rondini EA, Langohr IM, Fenton JI

    Institution

    Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.

    Source

    World journal of gastroenterology : WJG 18:7 2012 Feb 21 pg 627-36

    MeSH

    Adipokines
    Adiposity
    Animals
    Biological Markers
    Body Weight
    Caloric Restriction
    Colitis
    Diet, High-Fat
    Energy Metabolism
    Female
    Inflammation
    Male
    Mice
    Mice, Knockout
    Random Allocation
    Smad3 Protein
    Survival Rate

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22363133