Abstract

The 2009 influenza A virus (IAV) pandemic resulted from reassortment of avian, human and swine strains probably in pigs. To elucidate the role of viral genes in host adaptation regarding innate immune responses, we focussed on the effect of genes from an avian H5N1 and a porcine H1N1 IAV on infectivity and activation of porcine GM-CSF-induced dendritic cells (DC). The highest interferon type I responses were achieved by the porcine virus reassortant containing the avian polymerase gene PB2. This finding was not due to differential tropism since all viruses infected DC equally. All viruses equally induced MHC class II, but porcine H1N1 expressing the avian viral PB2 induced more prominent nuclear NF-κB translocation compared to its parent IAV. The enhanced activation of DC may be detrimental or beneficial. An over-stimulation of innate responses could result in either pronounced tissue damage or increased resistance against IAV reassortants carrying avian PB2.

Links

Publisher Full Text

Authors

Ocaña-Macchi M, Ricklin ME, Python S, Monika GA, Stech J, Stech O, Summerfield A

Institution

Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland.

Source

Virology 427:1 2012 May 25 pg 1-9

MeSH

Animals
Birds
Cell Line
Chick Embryo
Dendritic Cells
Dogs
Genes, Viral
Granulocyte-Macrophage Colony-Stimulating Factor
Host-Pathogen Interactions
Humans
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H5N1 Subtype
Interferon Type I
Major Histocompatibility Complex
Mice
NF-kappa B
Orthomyxoviridae Infections
Pandemics
Promoter Regions, Genetic
RNA Replicase
Reassortant Viruses
Swine
Swine Diseases
Viral Proteins
Virus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22365327