Unbound MEDLINE

Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells.

Abstract

The 2009 influenza A virus (IAV) pandemic resulted from reassortment of avian, human and swine strains probably in pigs. To elucidate the role of viral genes in host adaptation regarding innate immune responses, we focussed on the effect of genes from an avian H5N1 and a porcine H1N1 IAV on infectivity and activation of porcine GM-CSF-induced dendritic cells (DC). The highest interferon type I responses were achieved by the porcine virus reassortant containing the avian polymerase gene PB2. This finding was not due to differential tropism since all viruses infected DC equally. All viruses equally induced MHC class II, but porcine H1N1 expressing the avian viral PB2 induced more prominent nuclear NF-κB translocation compared to its parent IAV. The enhanced activation of DC may be detrimental or beneficial. An over-stimulation of innate responses could result in either pronounced tissue damage or increased resistance against IAV reassortants carrying avian PB2.

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  • Publisher Full Text
  • Authors

    Ocaña-Macchi M, Ricklin ME, Python S, Monika GA, Stech J, Stech O, Summerfield A

    Institution

    Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland.

    Source

    Virology 427:1 2012 May 25 pg 1-9

    MeSH

    Animals
    Birds
    Cell Line
    Chick Embryo
    Dendritic Cells
    Dogs
    Genes, Viral
    Granulocyte-Macrophage Colony-Stimulating Factor
    Host-Pathogen Interactions
    Humans
    Influenza A Virus, H1N1 Subtype
    Influenza A Virus, H5N1 Subtype
    Interferon Type I
    Major Histocompatibility Complex
    Mice
    NF-kappa B
    Orthomyxoviridae Infections
    Pandemics
    Promoter Regions, Genetic
    RNA Replicase
    Reassortant Viruses
    Swine
    Swine Diseases
    Viral Proteins
    Virus Replication

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22365327