Unbound MEDLINE

AA amyloid quantification in biopsy samples from the stomach.

Abstract

Amyloidosis is usually diagnosed through the histological examination of biopsy samples. However, its quantitative evaluation can be difficult. In this study, we immunochemically measured amyloid A (AA) proteins in biopsy samples taken from the stomachs of patients with AA amyloidosis. Samples were treated with guanidine and were subjected to an enzyme immunoassay for serum amyloid A. The results were compared with histological findings. All patients who tested negative for amyloid deposits had low values that clearly distinguished them from amyloid-positive patients. Among the amyloid-positive patients, the AA values correlated significantly with histological findings. This method may be useful for the quantitative evaluation of AA amyloidosis.

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  • Publisher Full Text
  • Authors

    Yamada T, Okuda Y

    Institution

    Department of Clinical Laboratory Medicine, Jichi Medical University, Yakushiji, Shimotsuke, Tochigi, Japan. yamadanji@jichi.ac.jp

    Source

    Annals of clinical and laboratory science 42:1 2012 pg 3-6

    MeSH

    Aged
    Aged, 80 and over
    Amyloidosis
    Biopsy
    Female
    Humans
    Immunoblotting
    Male
    Middle Aged
    Serum Amyloid A Protein
    Stomach

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22371903