Abstract

Glucagon-like peptide-1 (GLP-1) and leptin are anorectic hormones produced in the small intestine and white adipose tissue, respectively. Investigating how these hormones act together as an integrated anorectic signal is important to elucidate a mechanism to maintain energy balance. In the present study, coadministration of subthreshold GLP-1 and leptin dramatically reduced feeding in rats. Although coadministration of GLP-1 with leptin did not enhance leptin signal transduction in the hypothalamus, it significantly decreased phosphorylation of AMP-activated protein kinase (AMPK). In addition, coadministration of GLP-1 with leptin significantly increased proopiomelanocortin (POMC) mRNA levels. Considering that α-melanocortin stimulating hormone (α-MSH) is derived from POMC and functions through the melanocortin-4-receptor (MC4-R) as a key molecule involved in feeding reduction, the interaction of GLP-1 and leptin on feeding reduction may be mediated through the α-MSH/MC4-R system. As expected, the interaction of GLP-1 and leptin was abolished by intracerebroventricular preadministration of the MC4-R antagonists agouti-related peptide and SHU9119. Taken together, GLP-1 and leptin cooperatively reduce feeding at least in part via inhibition of AMPK following binding of α-MSH to MC4-R.

Links

Publisher Full Text

Authors

Poleni PE, Akieda-Asai S, Koda S, Sakurai M, Bae CR, Senba K, Cha YS, Furuya M, Date Y

Institution

Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan.

Source

Biochemical and biophysical research communications 420:1 2012 Mar 30 pg 36-41

MeSH

AMP-Activated Protein Kinases
Animals
Drug Interactions
Eating
Feeding Behavior
Glucagon-Like Peptide 1
Leptin
Male
Melanocyte-Stimulating Hormones
Rats
Rats, Wistar
Receptor, Melanocortin, Type 4
alpha-MSH

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22390932