Unbound MEDLINE

A natural antisense transcript regulates mucD gene expression and biofilm biosynthesis in Pseudomonas aeruginosa.

Abstract

Natural antisense transcripts (NATs) are naturally widespread and have very important functions in regulating expression of their target genes. In this report, we identified a novel NAT, designated mucD-AS, transcribed from the opposite strand ofmucD gene locus, which has a pivotal function in regulating the biosynthesis of alginate. For the biotechnological and medical significance in the study of alginate biosynthesis, we investigated the effect ofmucD-AS on mucD expression and alginate biosynthesis, and confirmed mucD-AS can induce biofilm formation ofP. aeruginosa significantly. Since alginate has a key function in the infection process and acts as virulence factor of P. aeruginosa, we speculate mucD-AS may have a regulatory function in this process.

Authors

Yang Z, Jin X, Rao X, Hu F

Source

Mikrobiologiia 80:6 pg 756-62

MeSH

Alginates
Bacterial Proteins
Base Sequence
Biofilms
Blotting, Northern
Gene Expression Regulation, Bacterial
Genes, Bacterial
Glucuronic Acid
Hexuronic Acids
Molecular Sequence Data
Pseudomonas aeruginosa
RNA, Antisense
Sigma Factor
Transcription, Genetic
Virulence Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22393760