Abstract

BACKGROUND
VKORC1, CYP2C9 and CYP4F2 are three critical genes associated with inter-individual variation of warfarin dose. Many dosing algorithms containing these gene polymorphisms and demographic characteristics have been set up for better use of warfarin. However, with distinct gene mutation frequencies among different ethnics, dosing algorithms differ greatly. For Chinese, related research just concentrate on Han Chinese, ignoring other Chinese ethnicities. This study aims to detect the popular polymorphisms in these three critical genes in Bai, Tibetan Chinese, to start the exploration of better use of warfarin in Chinese minorities.
METHODS
PCR-based methods were used to analyze VKORC1 3673G > A, CYP2C9*3, CYP4F2 rs2108622 C > T in Han, Bai and Tibetan Chinese.
RESULTS
The differences among the mutation frequencies of the studied genes in three ethnicities were not statistically significant. The frequency of A-allele of VKORC1 3673G > A was 92.8%, 90.2%, 90.8% in Bai, Tibetan, Han Chinese, respectively. The frequency of *3-allele in CYP2C9*3 was low in Bai (4.5%), Tibetan (2.8%) and Han Chinese (4.6%). Approximately one fourth of each ethnic had the mutant T-allele of CYP4F2 rs108622. However, Bai Chinese got statistically higher A-allele frequency of VKORC1 3673G > A than previously studied Han Chinese did.
CONCLUSIONS
Bai Chinese got significant higher A-allele frequency of VKORC1 3673G > A.

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Authors

Zeng WT, Zheng QS, Huang M, Cen HJ, Lai Y, Chen WY, Zhao LZ, Leng XY

Institution

School of Pharmaceutical Sciences, Sun Yat-sen University Guangzhou, China.

Source

Die Pharmazie 67:1 2012 Jan pg 69-73

MeSH

Adolescent
Adult
Aged
Aged, 80 and over
Alleles
Aryl Hydrocarbon Hydroxylases
Asian Continental Ancestry Group
Child
Cytochrome P-450 Enzyme System
DNA
DNA Primers
Female
Gene Frequency
Genotype
Humans
Male
Middle Aged
Mixed Function Oxygenases
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Tibet
Young Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22393834