Unbound MEDLINE

Extrachromosomal microDNAs and chromosomal microdeletions in normal tissues.

Abstract

We have identified tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mouse and human cell lines. These microDNAs are 200 to 400 base pairs long, are derived from unique nonrepetitive sequence, and are enriched in the 5'-untranslated regions of genes, exons, and CpG islands. Chromosomal loci that are enriched sources of microDNA in the adult brain are somatically mosaic for microdeletions that appear to arise from the excision of microDNAs. Germline microdeletions identified by the "Thousand Genomes" project may also arise from the excision of microDNAs in the germline lineage. We have thus identified a previously unknown DNA entity in mammalian cells and provide evidence that their generation leaves behind deletions in different genomic loci.

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  • Publisher Full Text
  • Authors

    Shibata Y, Kumar P, Layer R, Willcox S, Gagan JR, Griffith JD, Dutta A

    Institution

    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA.

    Source

    Science (New York, N.Y.) 336:6077 2012 Apr 6 pg 82-6

    MeSH

    5' Untranslated Regions
    Animals
    Base Pairing
    Base Sequence
    Brain
    Brain Chemistry
    Cell Line
    Cell Line, Tumor
    Chromosome Deletion
    Chromosomes, Human
    Chromosomes, Mammalian
    CpG Islands
    DNA Replication
    DNA, Circular
    Exons
    Germ Cells
    Heart
    Humans
    Liver
    Mice
    Mice, Inbred C57BL
    Microscopy, Electron
    Molecular Sequence Data
    Polymerase Chain Reaction
    Repetitive Sequences, Nucleic Acid

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    22403181