Unbound MEDLINE

Molecular epidemiologic characterization of Enterocytozoon bieneusi in HIV-infected persons in Benin City, Nigeria.

Abstract

Molecular characterization of Enterocytozoon bieneusi has led to better understanding of microsporidiosis transmission in humans. This study aimed to detect and genotype E. bieneusi in human immunodeficiency virus (HIV)-infected persons. Stool specimens were collected from 463 HIV-infected patients and analyzed for E. bieneusi by polymerase chain reaction (PCR) and DNA sequence analysis of the internal transcribed spacer. E. bieneusi was detected in 77 HIV patients. CD4 cell counts < 200 cells/μL was associated with E. bieneusi infection (P = 0.09). E. bieneusi was significantly associated with weight loss (P < 0.0001), diarrhea (P = 0.006), fever (P < 0.0001), not being married (P < 0.0001), and flush type of toilet (P = 0.0007). Six known genotypes of D, A, IV, CAF2, EbpA, and Peru 8 in 31, 22, 14, 2, 1, and 1 patients, respectively, five novel genotypes of E. bieneusi, and one infection with mixed genotypes were observed in this study. Three of the novel genotypes were genetically distant to the genotypes commonly found in humans.

Links

  • PMC Free PDF
  • PMC Free Full Text
  • FREE Publisher Full Text
  • Authors

    Akinbo FO, Okaka CE, Omoregie R, Dearen T, Leon ET, Xiao L

    Source

    The American journal of tropical medicine and hygiene 86:3 2012 Mar pg 441-5

    MeSH

    Adult
    CD4 Lymphocyte Count
    DNA, Fungal
    Enterocytozoon
    Feces
    Female
    Genotype
    HIV
    HIV Infections
    Humans
    Male
    Microsporidiosis
    Middle Aged
    Molecular Epidemiology
    Nigeria
    Phylogeny
    Risk Factors
    Sequence Analysis, DNA
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22403314