Unbound MEDLINE

Fabrication of gold nanoparticle assembled polyurethane microsphere template in trypsin immobilization.

Abstract

The separation, reusability and high catalytic activity of bioconjugate remain challenging task in proteins bound gold nanoparticles. A facile synthetic route for the fabrication of gold nanoparticle assembled polyurethane microsphere template and immobilization of trypsin on gold/polyurethane surface to form trypsin-nanogold-polyurethane bioconjugate was developed. The bioconjugate was characterized by X-ray diffraction, Field emission scanning electron microscopy, Transmission electron microscopy, UV-visible, Fourier transform infrared, Fluorescence and Circular dichroism spectroscopy. The catalytic studies confirmed retention of approximately 40% of its original activity even after eight consecutive reaction cycles. The bioconjugate is also very effective for its separation from the reaction medium and exhibited significant enhanced stability over a wide range of pH and temperature compared to free trypsin. These findings clearly demonstrate that trypsin immobilized gold nanoparticle assembled polyurethane microsphere acts as an excellent recyclable biocatalyst with enzyme-specific biocompatibility.

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  • Publisher Full Text
  • Authors

    Kotal M, Srivastava SK, Maiti TK

    Source

    Journal of nanoscience and nanotechnology 11:11 2011 Nov pg 10149-57

    MeSH

    Bioreactors
    Enzyme Stability
    Enzymes, Immobilized
    Equipment Reuse
    Gold
    Hydrogen-Ion Concentration
    Metal Nanoparticles
    Microspheres
    Nanoconjugates
    Polyurethanes
    Spectrometry, Fluorescence
    Spectroscopy, Fourier Transform Infrared
    Trypsin

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22413358