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16S rRNA gene sequence analysis of a Brucella melitensis infection misidentified as Bergeyella zoohelcum.

Abstract

Misidentification of Brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate from a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieux Inc., Durham, NC, USA). Because of this identification ambiguity by the two automated bacterial identification systems we performed 16S rRNA sequencing and serotyping of the isolate and confirmed it as a Brucella spp. Combining the sequence data with the Vitek 2 system data we conclude that the infection was caused by B. melitensis.

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  • Publisher Full Text

    • Authors

    Dash N, Panigrahi D, Al-Zarouni M, Mishra S

    Institution

    College of Medicine, University of Sharjah, United Arab Emirates. ndash@sharjah.ac.ae

    Source

    Journal of infection in developing countries 6:3 2012 Mar pg 283-6

    MeSH

    Adult
    Automation
    Bacteremia
    Bacterial Typing Techniques
    Brucella melitensis
    Brucellosis
    DNA, Bacterial
    Diagnostic Errors
    Flavobacteriaceae
    Flavobacteriaceae Infections
    Genes, rRNA
    Humans
    Male
    RNA, Ribosomal, 16S
    Reagent Kits, Diagnostic
    Sequence Analysis, DNA
    Serotyping

    Pub Type(s)

    Case Reports
    Journal Article

    Language

    eng

    PubMed ID

    22421611