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- Authors
- Koizumi N
- Nakajima C
- Harunari T
- Tanikawa T
- Tokiwa T
- Uchimura E
- Furuya T
- Mingala CN
- MESH
- Bacteriological Techniques
- Genes, rRNA
- Humans
- Leptospira
- Leptospirosis
- Nucleic Acid Amplification Techniques
- RNA, Ribosomal, 16S
- Sensitivity and Specificity
- Specimen Handling
- Temperature
- Urine
A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine.
Abstract
We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.
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Authors
Koizumi N, Nakajima C, Harunari T, Tanikawa T, Tokiwa T, Uchimura E, Furuya T, Mingala CN, Villanueva MA, Ohnishi M, Suzuki Y
Institution
Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan. nkoizumi@nih.go.jp
Source
Journal of clinical microbiology 50:6 2012 Jun pg 2072-4MeSH
Bacteriological TechniquesGenes, rRNA
Humans
Leptospira
Leptospirosis
Nucleic Acid Amplification Techniques
RNA, Ribosomal, 16S
Sensitivity and Specificity
Specimen Handling
Temperature
Urine
Pub Type(s)
Comparative StudyEvaluation Studies
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
22422858