Unbound MEDLINE

Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells.

Abstract

Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream molecules of MEKK1. Then, hBSM cell overexpressing MEKK1 were exposed to apigenin (50 microM). Our data indicated that apigenin inhibited significantly activation/phosphorylation of MAPKs and migration of hBSM cells induced by MEKK1 overexpression. Besides, apigenin inhibited actin polymerization, which underlines muscle contraction and cell migration. The results suggest that apigenin inhibits activation of MAPKs and thereby the cell migration. The mechanism might be that apigenin blocks signal transmission from MEKK1 to MAPKs.

Authors

Liu Q, Chen X, Yang G, Min X, Deng M

Source

Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al 35:3 2011 Dec pg 71-9

MeSH

Adenoviridae
Animals
Apigenin
Cell Movement
Cells, Cultured
Humans
Immunoblotting
MAP Kinase Kinase Kinase 1
Mice
Mitogen-Activated Protein Kinases
Myocytes, Smooth Muscle
Phosphorylation
Signal Transduction
Urinary Bladder

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22423483