Abstract

BACKGROUND
Neoadjuvant chemoradiotherapy is recommended in the treatment of locally advanced rectal cancer. Studies have suggested that chemoradiotherapy adversely affects anorectal function. However, the functional implication and the underlying neuromyogenic changes involved in radiation-induced damage are poorly understood.
OBJECTIVE
This study evaluated the functional changes following chemoradiotherapy on the internal anal sphincter.
DESIGN AND PATIENTS
This article describes an in vitro study on the internal anal sphincter collected from patients undergoing abdominoperineal resection or proctectomy. Five patients were treated by surgery alone (control group), and 6 received preoperative chemoradiotherapy (treatment group). Sphincter strips were mounted in organ bath, and the responses to electrical field stimulation and drugs were monitored.
SETTINGS
The study was performed at the University of Oxford.
MAIN OUTCOME MEASURES
The end points of this study were to investigate whether chemoradiotherapy has any significant effects on internal anal sphincter function and, subsequently, to establish the type of injury induced.
RESULTS
Chemoradiotherapy strips developed similar tone, but significantly lower spontaneous activity (p = 0.001) than controls. Electrical field stimulation induced relaxation, followed by contraction. At 50 Hz, electrical field stimulation produced 25.6 ± 4.9% (mean ± SE) of maximum relaxation followed by a contraction of 5.5 ± 0.9% of basal tone in chemoradiotherapy strips i9n comparison with 47.0 ± 6.2% (p = 0.009) and 17.7 ± 4.0% (p = 0.007) in controls. Relaxation was significantly attenuated by N-nitro-L-arginine. Significant differences were found in responses to carbachol (p = 0.018) and phenylephrine (p = 0.022), but not to sodium nitroprusside.
LIMITATIONS
This work was limited by the relatively small number of patients enrolled, because of the difficulty of finding human tissue for laboratory studies, and the lack of long-term results.
CONCLUSIONS
Chemoradiotherapy significantly impairs internal anal sphincter function and intrinsic nerves seem more susceptible than smooth muscle. The exclusion of anal canal from the radiation field is recommended, when oncologically safe.

Links

Publisher Full Text

Authors

Lorenzi B, Brading AF, Martellucci J, Cetta F, Mortensen NJ

Institution

Department of Surgery, University of Siena, Siena, Italy. bruno.lorenzi@yahoo.it

Source

Diseases of the colon and rectum 55:4 2012 Apr pg 465-72

MeSH

Adult
Aged
Aged, 80 and over
Anal Canal
Arginine
Carbachol
Case-Control Studies
Chemoradiotherapy
Electric Stimulation
Female
Humans
Male
Middle Aged
Neoadjuvant Therapy
Phenylephrine
Rectal Neoplasms

Pub Type(s)

In Vitro
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22426272