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Expression and distribution of aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and bes-gluconate-sucrose solutions. Study of their correlation with water content.

Abstract

Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0 degree C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P less than 0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.

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  • Publisher Full Text
  • Authors

    Miszczuk G, Mediavilla MG, Pizarro MD, Tiribelli C, Rodríguez J, Mamprin ME

    Source

    Cryo letters 33:1 pg 75-85

    MeSH

    Alkanesulfonic Acids
    Animals
    Aquaporins
    Cell Size
    Cell Survival
    Cold Temperature
    Cryopreservation
    Gene Expression
    Gluconates
    Hepatocytes
    Immunohistochemistry
    Liver
    Male
    Microscopy, Confocal
    Organ Preservation Solutions
    Osmolar Concentration
    Rats
    Rats, Wistar
    Sucrose
    Water

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22434125