Expression and distribution of aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and bes-gluconate-sucrose solutions. Study of their correlation with water content.

Abstract

Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0 degree C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P less than 0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.

Links

Publisher Full Text

Authors

Miszczuk G, V MG, Pizarro MD, Tiribelli C, Rodríguez J, Mamprin ME

Institution

Centro Binacional (Argentina-Italia) de Investigaciones en Criobiología Clínica y Aplicada (CAIC) Universidad Nacional de Rosario Avda, Rosario, Argentina.

Source

Cryo letters 33:1 pg 75-85

MeSH

Alkanesulfonic Acids
Animals
Aquaporins
Cell Size
Cell Survival
Cold Temperature
Cryopreservation
Gene Expression
Gluconates
Hepatocytes
Immunohistochemistry
Liver
Male
Microscopy, Confocal
Organ Preservation Solutions
Osmolar Concentration
Rats
Rats, Wistar
Sucrose
Water

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22434125