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Differential motion dynamics of synaptic vesicles undergoing spontaneous and activity-evoked endocytosis.

Abstract

Synaptic vesicle exo- and endocytosis are usually driven by neuronal activity but can also occur spontaneously. The identity and differences between vesicles supporting evoked and spontaneous neurotransmission remain highly debated. Here we combined nanometer-resolution imaging with a transient motion analysis approach to examine the dynamics of individual synaptic vesicles in hippocampal terminals under physiological conditions. We found that vesicles undergoing spontaneous and stimulated endocytosis differ in their dynamic behavior, particularly in the ability to engage in directed motion. Our data indicate that such motional differences depend on the myosin family of motor proteins, particularly myosin II. Analysis of synaptic transmission in the presence of myosin II inhibitor confirmed a specific role for myosin II in evoked, but not spontaneous, neurotransmission and also suggested a functional role of myosin II-mediated vesicle motion in supporting vesicle mobilization during neural activity.

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  • Publisher Full Text
  • Authors

    Peng A, Rotman Z, Deng PY, Klyachko VA

    Institution

    Department of Cell Biology and Physiology, Department of Biomedical Engineering, Center for the Investigation of Membrane Excitability Diseases, Washington University, St. Louis, MO 63110, USA.

    Source

    Neuron 73:6 2012 Mar 22 pg 1108-15

    MeSH

    Analysis of Variance
    Animals
    Animals, Newborn
    Azepines
    Biophysics
    Cells, Cultured
    Electric Stimulation
    Endocytosis
    Enzyme Inhibitors
    Excitatory Postsynaptic Potentials
    Fluorescent Dyes
    Heterocyclic Compounds with 4 or More Rings
    Hippocampus
    Microscopy, Fluorescence
    Neurons
    Nonlinear Dynamics
    Organ Culture Techniques
    Patch-Clamp Techniques
    Rats
    Sodium Channel Blockers
    Statistics, Nonparametric
    Synapses
    Synaptic Vesicles
    Tetrodotoxin
    Time Factors

    Pub Type(s)

    In Vitro
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22445339