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Bacterial flora concurrent with Helicobacter pylori in the stomach of patients with upper gastrointestinal diseases.

Abstract

AIM
To investigate the non-Helicobacter pylori (H. pylori) bacterial flora concurrent with H. pylori infection.
METHODS
A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H. pylori bacterial isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
RESULTS
A total of 201 non-H. pylori bacterial isolates were cultivated from 67 (65.0%) of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothia and Staphylococcus, which differed from the predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group (100% vs 42.9%, P < 0.001). Six bacterial species with urease activity (Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacterium spp. and Klebsiella pneumoniae) were also isolated.
CONCLUSION
There is a high prevalence of the non-H. pylori bacteria concurrent with H. pylori infection, and the non-H. pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.

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    • Authors

    Hu Y, He LH, Xiao D, Liu GD, Gu YX, Tao XX, Zhang JZ

    Institution

    State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

    Source

    World journal of gastroenterology : WJG 18:11 2012 Mar 21 pg 1257-61

    MeSH

    Bacterial Proteins
    Biopsy
    Helicobacter Infections
    Helicobacter pylori
    Humans
    Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    Stomach
    Stomach Diseases
    Urease

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22468090