Abstract

A novel method was developed and single-laboratory validated for the determination of free pantothenic acid (vitamin B5) in a wide range of infant and adult fortified food products. The method combines simple sample preparation and chromatographic analysis using ultra-performance LC coupled to tandem MS with positive electrospray ionization. Pantothenic acid was quantified using [13C6, 15N2]-pantothenic acid as an internal standard. Calibration curves were linear between 0.08 and 1.2 microg/mL (r2 = 0.9998), and average recovery varied between 95 and 106%. The method exhibited overall RSD(r) of 1.1% and RSD intermediate reproducibility from 2.5 to 6.0% in infant formulas and cereals. Comparison of results between total and free pantothenic acid showed that the analysis of free pantothenic acid gave a good estimation of total pantothenic acid in the range of products analyzed. The method provides reliable free pantothenic acid results in a wide range of fortified foods (infant and adult nutritionals, cereal products and beverages), and shows good correlation with the microbiological method AOAC Official Method 992.07. It is a more selective, faster, and robust alternative to microbiological determination.

Links

Publisher Full Text

Authors

Andrieux P, Fontannaz P, Kilinc T, Giménez EC

Institution

Nestlé Research Center, Vers-chez-les-blanc, CH-1000 Lausanne 26, Switzerland.

Source

Journal of AOAC International 95:1 pg 143-8

MeSH

Algorithms
Biological Assay
Buffers
Calibration
Chromatography, High Pressure Liquid
Food, Fortified
Indicators and Reagents
Infant Food
Lactobacillus plantarum
Pantothenic Acid
Reference Standards
Regression Analysis
Reproducibility of Results
Solutions
Solvents
Tandem Mass Spectrometry

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22468352