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Evaluation of microwave-assisted extraction for the analysis of organophosphorus and pyrethroid pesticides in green onions.

Abstract

The aim of this study was to evaluate the efficiency of microwave-assisted extraction (MAE) for the analysis of organophosphorus (OP) and pyrethroid (PYR) pesticides in green onions by GC/MS. We optimized MAE extraction solvent, temperature, and time by using a certified reference material. As a result, the concentrations of two OP and two PYR target pesticides obtained by MAE with acetonitrile at 110 degrees C for 10 min were in good agreement with certified concentrations and comparable to the results by homogenization used as reference extraction technique. When the recovery test, performed by spiking the target pesticides into blank samples (5.0 g), was carried out with our optimized MAE conditions, mean recoveries of 16 OP and 10 PYR pesticides were 72-108% for a 1.0 pg spiking level and 70-119% for a 0.2 microg level. These results were acceptable according to the validation guideline for testing method of agricultural chemicals in food by Ministry of Health, Labour, and Welfare in Japan. The results suggested that MAE can be used for the analysis of OP and PYR pesticides in green onions.

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  • Authors

    Otake T, Kuroda Y, Aoyagi Y, Yarita T

    Institution

    National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Umezono, Tsukuba, Ibaraki 305-8563, Japan. t-ootake@aist.go.jp

    Source

    Journal of AOAC International 95:1 pg 232-7

    MeSH

    Calibration
    Diazinon
    Fenitrothion
    Food Analysis
    Gas Chromatography-Mass Spectrometry
    Indicators and Reagents
    Isotope Labeling
    Limit of Detection
    Microwaves
    Onions
    Organophosphorus Compounds
    Permethrin
    Pesticides
    Pyrethrins
    Reference Standards
    Solid Phase Extraction
    Solvents

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22468365