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Validation of the Delvotest SP NT DA. Performance Tested Method 011101.

Abstract

Delvotest SP NT DA is designed to test milk for the presence of antibacterial substances, such as antibiotics. The test is made of an agar gel containing bacterial spores and a color indicator. The milk sample is added onto the agar gel, and the test is incubated at 64 degrees C. The principle of the test is based on the diffusion of possible inhibitory substances that may be present in the milk sample into agar. This reduces growth and acid production by the test organism, and delays or prevents the agar from changing color from purple to yellow. The Delvotest Accelerator is an automated system in which the plates containing the milk to be analyzed are placed for incubation. The Accelerator automatically detects the end of the incubation and reads the results. A sample containing antibiotic will be noted as "positive." A sample without antibiotics or with antibiotics at concentrations below detection level will be noted as "negative." The present report includes all technical details about the Delvotest SP NT DA, and the results of the validation study. The validation study demonstrates that the Delvotest SP NT DA conforms to the product performance claims and confirms the robustness of the test. The Delvotest SP NT DA is, therefore, granted Performance Tested Method certification.

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  • Publisher Full Text
  • Authors

    Hennart SL, Faragher J

    Source

    Journal of AOAC International 95:1 pg 252-60

    MeSH

    Animal Feed
    Animals
    Anti-Bacterial Agents
    Bacteria
    Cattle
    Cephapirin
    Colony Count, Microbial
    Computers
    Drug Residues
    Freezing
    Indicators and Reagents
    Milk
    Penicillins
    Reagent Kits, Diagnostic
    Reference Standards
    Reproducibility of Results
    Software
    Specimen Handling

    Pub Type(s)

    Journal Article
    Validation Studies

    Language

    eng

    PubMed ID

    22468368