Abstract

AIM
This study investigated the levels of Apurinic/Apyrimidinic Endonuclease (APEX1) in gallbladder carcinoma (CaGB) tissue and co-related these levels with various clinicopathological parameters.
PATIENTS AND METHODS
Twenty cases of CaGB and cholelithiasis were included in the study. Western blot analysis of APEX1 protein was performed using actin as the reference point. Densitometric analysis and the integrated density value (IDV) of APEX1 protein samples were determined. The ratio of IDV of APEX1/actin was determined.
RESULTS
The mean IDV ratio of APEX1 in CaGB was 0.63±0.33 and 0.45±0.19 in cholelithiasis. The mean IDV ratio of a variant of APEX1 (ΔAPEX1) in CaGB was 0.50±0.09, whereas it was 0.40±0.16 in cholelithiasis. Calculating the mean IDV ratio of total APEX (APEX1+ΔAPEX1) in CaGB was 1.13±0.31 whereas in cholelithiasis, 0.85±0.23. The differences were statistically significant (p<0.05).
CONCLUSION
A significant correlation was found between the relative expressions of APEX1 in cancer as compared to that in cholelithiasis patients. There was significant association between APEX1 expression and perineural invasion. A variant of APEX1 correlated with tumor infiltration. Hence APEX1 may be of use as a prognostic marker in patients with CaGB.

Links

Publisher Full Text

Authors

Shukla VK, das PC, Dixit R, Bhartiya SK, Basu S, Raman MJ

Institution

Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. vkshuklabhu@gmail.com

Source

Anticancer research 32:4 2012 Apr pg 1489-92

MeSH

Adult
Aged
DNA Repair
DNA-(Apurinic or Apyrimidinic Site) Lyase
Female
Gallbladder Neoplasms
Humans
Male
Middle Aged

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22493391